Three-Dimensional Structure of Human Follicle-Stimulating Hormone

Three-dimensional structure of human follicle-stimulating hormone.

The crystal structure of a betaThr26Ala mutant of human follicle-stimulating hormone (hFSH) has been determined to 3.0 A resolution. The hFSH mutant was expressed in baculovirus-infected Hi5 insect cells and purified by affinity chromatography, using a betahFSH-specific monoclonal antibody. The betaThr26Ala mutation results in elimination of the betaAsn24 glycosylation site, yielding protein mo...

Overnight radioimmunoassay of human follicle-stimulating hormone.

A radioimmunoassay procedure for follicle-stimulating hormone in serum has been developed in which the assay mixture is incubated at 37#{176}C for a total of 22 h, rather than at 4#{176}C for 4-7 days. The withinassay coefficients of variation for the 37#{176}C and 4#{176}C methods, respectively, are 7.7% and 5.0%, while the between-assay variations are 13.8% and 9.9%. Comparison of values for ...

Follicle-stimulating hormone and human spermatogenesis.

The role of follicle-stimulating hormone (FSH) in the control of spermatogenesis is not well established in any species, including man. We studied the effect of an experimentally-induced, selective FSH deficiency on sperm production in normal men. After a 3-mo control period, five normal men received testosterone enanthate (T) 200 mg i. m. weekly to suppress luteinizing hormone (LH) and FSH, un...

Dissociation of human follicle-stimulating hormone. Comparison with luteinizing hormone.

Rat testis tissue receptor assays were utilized to study the kinetics of dissociation of human follicle-stimulating hormone (hFSH) and luteinizing hormone (hLH) under varying conditions of urea concentration and pH. In these competitive protein binding assays, 125I-hFSH and 125I-hLH were the radioligands and hormone dissociation was followed by a decrease in the ability of the dissociating horm...

Follicle-stimulating hormone